Journal: International Journal of Molecular Sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. ( A ) GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after 24 h of the in vitro addition of different concentrations of the adiponectin receptor agonist adipoRon treatment (0 µM, 0.1 µM, 1 µM, and 10 µM). ( B ) Histogram analysis of Western Blot gray values corresponding to changes in GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after in vitro treatment with different concentrations of the adiponectin receptor agonist adipoRon (0 µM, 0.1 µM, 1 µM, and 10 µM). ( C ) Levels of glucose content in granulosa cells after 24 h of treatment with different concentrations of the adiponectin receptor agonist AdipoRon in vitro (0 µM, 1 µM, and 10 µM). The error bars represent means ± SEM ( n = 3, each group). ns represents no significance. * Statistically significant values (* p < 0.05; ** p < 0.01; **** p < 0.0001).

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc-46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-rabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: In Vitro, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Transcriptome data and correlation analysis of the expression of glucose transporters and the adipokine system in rat ovaries. ( A ) Volcanic map analysis of transcriptome data. Blue: represents no significant difference. ( B ) KEGG pathway analysis; ( C ) correlation analysis of genes related to the glucose metabolism signaling pathway and adipokine signaling pathway. ( D ) Gene transcription levels of Slc2a1 , Slc2a2 , Slc2a3 , Slc2a4 , Adipor1 , and Adipor2 in the ovaries of rats in the eCG-treated and blank control groups. ( E ) Protein levels of GLUT1, GLUT2, GLUT3, GLUT4, AdipoR1, and AdipoR2 in the ovaries of rats in the eCG-treated and blank control groups. The error bars represent means ± SEM ( n = 3, each group). * Statistically significant values (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001); ns represents no significance.

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc-46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-rabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: Expressing, Control

Journal: Journal of Neuroinflammation

Article Title: Changes in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies

doi: 10.1186/s12974-024-03060-4

Figure Lengend Snippet: Tau pathology switches the energy metabolism to fatty acid oxidation. A-B Immunofluorescence staining of neuron-specific glucose transporter 3 (GLUT3) in the medulla oblongata from control and transgenic animals. Representative images of GLUT3 (green), NeuN (red), and DAPI staining (blue). C Representative images of 8-month-old control and transgenic animals. Scale bar 20 μm. D . Quantification of GLUT3 mean fluorescence intensity in brain tissue. Quantification of relative fluorescent intensity showed a decrease of GLUT3 in transgenic animals (8-months old animals: CN: 25.07 ± 1.43, Tg: 20.91 ± 0.54; p = 0.035; 10-month-old animals: CN: 28.37 ± 1.03, Tg: 22.36 ± 1.67; p = 0.022; n = 5; mean ± SD, student’s t-test with p-value). E Changes of long-chain, hydroxylated, short-chain ACs and free carnitine in brain tissue of control and transgenic rats (data are presented as median intensities with 95% confidence intervals)

Article Snippet: Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000, Wako), polyclonal rabbit anti-GLUT3 primary antibody (1:100, Invitrogen, Massachusetts, USA), and monoclonal mouse anti-NeuN primary antibody (1:1000, Abcam, Waltham, MA, USA).

Techniques: Immunofluorescence, Staining, Transgenic Assay, Fluorescence

Journal: Cell reports

Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability

doi: 10.1016/j.celrep.2023.113374

Figure Lengend Snippet: (A) Volcano plot derived from bulk RNA-seq data collected from PDGCs lentivirally infected with the SCR or CD97 shRNA (n = 3 biological replicates for each). Genes involved in canonical glycolysis and glucose transport are represented as large yellow points. Genes involved in the TCA cycle and OXPHOS (cytochrome oxidase subunits) are represented as large green points. CD97 is represented by an orange point. (B) The top ten enriched and depleted pathways determined by the largest fold enrichment among downregulated genes using GO PANTHER pathway enrichment analysis. Stars indicate metabolic pathways. (C) Correlation matrix from bulk RNA-seq data collected from PDGCs following knockdown or overexpression of CD97 shows high correlation between CD97 and glycolysis-related genes and anti-correlation with TCA cycle-related genes. HK2 (hexokinase 2) and SLC2A3 (glucose transporter 3 [GLUT3]) transcripts are both included because they have been implicated in Warburg metabolism. (D) Bar graph showing decreased lactate production after knockdown of CD97 (n = 6 per PDGC; two-way ANOVA F 1,15 = 27.24, p < 0.001) and increased lactate production after CD97 overexpression in PDGCs (n = 3 per PDGC; two-way ANOVA F 1,6 = 26.07, p < 0.01). (E) Steady-state metabolomic data reveal depletion of glycolytic metabolites after knockdown of CD97 in PDGCs (PN [proneural], n = 2–3 [one replicate was removed for technical reasons]; CL [classical], n = 3). (F) Depleted (red) and enriched (green) heavy-labeled glycolytic and TCA cycle metabolites after a heavy-labeled glucose tracing experiment. (G) Representative Seahorse Cell Energy Phenotype graph showing OCR and ECAR changes before (baseline) and after (maximal) addition of mitochondrial stressors. (H and I) Bar graphs quantifying baseline and maximal ECAR (n = 6 per PDGC; baseline: two-way ANOVA F 1,10 = 14.06; **p < 0.01; maximal: two-way ANOVA F 1,10 = 17.87, p < 0.01). (J and K) Bar graphs quantifying baseline and maximal OCR (n = 6 per PDGC; baseline: two-way ANOVA F 1,10 = 2.820, p > 0.05; maximal: two-way ANOVA F 1,10 = 5.474; *p < 0.05). Error bars indicate SEM.

Article Snippet: Rabbit polyclonal anti-glucose transporter GLUT3 antibody (ab15311) , Abcam , AB15311; RRID:AB_301846.

Techniques: Derivative Assay, RNA Sequencing Assay, Infection, shRNA, Over Expression, Labeling

Journal: Cell reports

Article Title: The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability

doi: 10.1016/j.celrep.2023.113374

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-glucose transporter GLUT3 antibody (ab15311) , Abcam , AB15311; RRID:AB_301846.

Techniques: Recombinant, Modification, Disruption, Activation Assay, Knock-Out, shRNA

Journal: bioRxiv

Article Title: Changes in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies

doi: 10.1101/2023.09.05.556321

Figure Lengend Snippet: A-B Immunofluorescence staining of neuron-specific glucose transporter 3 (GLUT3). Representative images of GLUT3 (green), NeuN (red), and DAPI staining in brain tissue from control and transgenic animals. C Representative images of 8-month-old control and transgenic animals. Scale bar 20 µm. D . Quantification of GLUT3 mean fluorescence intensity in brain tissue. Quantification of relative fluorescent intensity showed a decrease of GLUT3 in transgenic animals (8-months old animals: CN: 25.07 ± 1.43, Tg: 20.91 ± 0.54; p= 0.035; 10-month-old animals: CN: 28.37 ± 1.03, Tg: 22.36 ± 1.67; p= 0.022; n= 5; mean ± SD, student’s t-test with p-value). E Changes of long-chain, hydroxylated, short-chain ACs and free carnitine in brain tissue of control and transgenic rats (data are presented as median intensities with 95% confidence intervals).

Article Snippet: Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000, Wako), polyclonal rabbit anti-GLUT3 primary antibody (1:100, Invitrogen, Massachusetts, USA), and monoclonal mouse anti-NeuN primary antibody (1:1000, Abcam, Waltham, MA, USA).

Techniques: Immunofluorescence, Staining, Transgenic Assay, Fluorescence

Journal: Life

Article Title: Expression of Glucose Transporters 1 and 3 in the Placenta of Pregnant Women with Gestational Diabetes Mellitus

doi: 10.3390/life13040993

Figure Lengend Snippet: mRNA expression of GLUT1 ( A ) and GLUT3 ( B ). The results show that there was no significant difference between the placentas of pregnant women with gestational diabetes mellitus (GDM) and those of healthy pregnant women.

Article Snippet: Rabbit anti-GLUT1 polyclonal antibody (Biorbyt, orb157188) and rabbit anti-GLUT3 polyclonal antibody (Biorbyt, orb10727) were used as the primary antibodies.

Techniques: Expressing

Journal: Life

Article Title: Expression of Glucose Transporters 1 and 3 in the Placenta of Pregnant Women with Gestational Diabetes Mellitus

doi: 10.3390/life13040993

Figure Lengend Snippet: Protein expression of GLUT1 ( A ) and GLUT3 ( B ) on chorionic villi of the placenta. The results showed a significant increase in the protein expressions of GLUT1 and GLUT 3 in the placentas of pregnant women with GDM compared to the corresponding values in the placentas of healthy pregnant women. (*) p ≤ 0.05. (**) p ≤ 0.01.

Article Snippet: Rabbit anti-GLUT1 polyclonal antibody (Biorbyt, orb157188) and rabbit anti-GLUT3 polyclonal antibody (Biorbyt, orb10727) were used as the primary antibodies.

Techniques: Expressing

Journal: Life

Article Title: Expression of Glucose Transporters 1 and 3 in the Placenta of Pregnant Women with Gestational Diabetes Mellitus

doi: 10.3390/life13040993

Figure Lengend Snippet: Results of immunohistochemical (IHC) staining of GLUT1 and GLUT3 in placenta sections. ( A , B ) IHC images of placenta sections, scale bar 20 µm (-ve control: without primary antibodies). ( a , b ) Statistical analysis of IHC results. The results demonstrated that the GLUT1 and GLUT3 were significantly increased in the placentas of pregnant women with GDM compared to healthy pregnant women (***) p ≤ 0.001.

Article Snippet: Rabbit anti-GLUT1 polyclonal antibody (Biorbyt, orb157188) and rabbit anti-GLUT3 polyclonal antibody (Biorbyt, orb10727) were used as the primary antibodies.

Techniques: Immunohistochemical staining, Immunohistochemistry, Control